FINE-STRUCTURE MAPPING AND MUTATIONAL STUDIES OF GENE CONTROLLING YEAST CYTOCHROME c
نویسندگان
چکیده
منابع مشابه
Mapping and gene conversion studies with the structural gene for iso-1-cytochrome C in yeast.
We have investigated the order of the four genes cyc1, rad7, SUP4, and cdc8 which form a tightly linked cluster on the right arm of chromosome X in the yeast Saccharomyces cerevisiae. Crossing over and coconversion data from tetrad analysis established the gene order to be centromere-cyc1-rad7-SUP4. Also cdc8 appeared to be distal to SUP4 on the basis of crossovers that were associated with con...
متن کاملThe mutational alteration of the primary structure of yeast iso-1-cytochrome c.
A benzidine-HzOz staining procedure and genetic tests were used for the detection of cytochrome c-deficient mutants of the cyl, cy2, cy3, cy4, and cy6 loci. The chromosomal gene, CY1, was shown to encode the primary structure of iso-l-cytochrome c from bakers’ yeast. Sixteen cyl mutants were isolated after examining over 1 million colonies. These cyl mutants could be categorized as follows: (a)...
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A large number of amdS mutants altered in acetamide utilization have been used to construct a fine-structure map of the amdS locus. The mutagen diepoxyoctane generated most of the deletion strains used for mapping. A minimum of 14 sites within the amdS gene were found. Biochemical analysis of amdS mutants defined the extent of the probable coding region. A new mutant, amd-205, which did not pro...
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The X-ray mapping procedure of Manney and Mortimer (1964) is the most widely applicable and convenient method for fine structure analysis in yeast, but suffers the disadvantage that suitable X-ray machines or gamma ray sources are very expensive. Although many other recombinogens are known, none gives a linear dose-response like X-rays and few are as convenient or give as reproducible results. ...
متن کاملStructural Characteristics of Stable Folding Intermediates of Yeast Iso-1-Cytochrome-c
Cytochrome-c (cyt-c) is an electron transport protein, and it is present throughout the evolution. More than 280 sequences have been reported in the protein sequence database (www.uniprot.org). Though sequentially diverse, cyt-c has essentially retained its tertiary structure or fold. Thus a vast data set of varied sequences with retention of similar structure and fun...
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ژورنال
عنوان ژورنال: Genetics
سال: 1969
ISSN: 1943-2631
DOI: 10.1093/genetics/62.1.9